Blar i forfatter "Haug, Bjørn Helge"

Viser treff 1-4 av 4

    • Conditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastoma 

      Henriksen, Jørn Remi; Haug, Bjørn Helge; Büchner, Jochen; Tømte, Ellen; LØkke, cecilie; Flaegstad, Trond; Einvik, christer (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      Background: Neuroblastoma is a childhood cancer derived from immature cells of the sympathetic nervous system. The disease is clinically heterogeneous, ranging from neuronal differentiated benign ganglioneuromas to aggressive metastatic tumours with poor prognosis. Amplification of the MYCN oncogene is a well established poor prognostic factor found in up to 40% of high risk neuroblastomas. Using ...

    • Exosome-like extracellular vesicles from MYCN-amplified neuroblastoma cells contain oncogenic miRNAs 

      Haug, Bjørn Helge; Hald, Øyvind; Utnes, Peter; Roth, Sarah A; Løkke, Cecilie; Flægstad, Trond; Einvik, Christer (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      <p><i>Background:</i> In recent years, evidence has accumulated indicating that both normal and cancer cells communicate via the release and delivery of macromolecules packed into extracellular membrane vesicles. <p><i>Materials and Methods:</i> We isolated nano-sized extracellular vesicles from MYCN-amplified neuroblastoma cell lines using ultracentrifugation and exosome precipitation (Exoquick) ...

    • MYCN-regulated miRNAs Inhibit Secretion of the Tumor Suppressor DICKKOPF-3 (DKK3) in Neuroblastoma 

      Haug, Bjørn Helge; Henriksen, Jørn Remi; Buechner, Jochen; Kogner, Per; Martinsson, Tommy; Flægstad, Trond; Sveinbjørnsson, Baldur; Einvik, Christer (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      The MYCN oncogene is frequently amplified in neuroblastoma. It is one of the most consistent markers of a bad prognosis for this disease. Dickkopf-3 (DKK3) is a secreted protein of the Dickkopf family of Wnt regulators. It functions as a tumor suppressor in a range of cancers, including neuroblastoma. MYCN was recently found to downregulate DKK3 mRNA. In this study, we show that MYCN knockdown in ...

    • Tumour-suppressor microRNAs let-7 and mir-101 target the proto-oncogene MYCN and inhibit cell proliferation in MYCN-amplified neuroblastoma 

      Buechner, Jochen; Tømte, Ellen; Haug, Bjørn Helge; Henriksen, Jørn Remi; Løkke, Cecilie; Flægstad, Trond; Einvik, Christer (Journal article; Tidsskriftartikkel; Peer reviewed, 2011-06-07)
      BACKGROUND: MicroRNAs (miRNAs) regulate expression of many cancer-related genes through posttranscriptional repression of their mRNAs. In this study we investigate the proto-oncogene MYCN as a target for miRNA regulation.<p> <p>METHODS: A luciferase reporter assay was used to investigate software-predicted miRNA target sites in the 30 -untranslated region (30 UTR) of MYCN. The miRNAs were ...